Sufficient reagents provided for 40 reactions based on a 50 µl reaction volume. Applied biosystems amplitaq gold dna polymerase ld is a hot start pcr enzyme provided in an inactive state allowing for room temperature set up and minimizing non specific amplification. The mix is optimized for sybr green re actions and contains sybr green i dye amplitaq gold dna polymerase dntps with dutp passive reference and optimized buffer components.
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