Sybr Green Qpcr Mix

Sybr green master mixes are 2x concentrated and contain dntps mgcl 2 and dna polymerase.

Sybr green qpcr mix.

Dye based quantitative pcr qpcr uses real time fluorescence of a double stranded dna dsdna binding dye most commonly sybr green i to measure dna amplification during each cycle of a pcr. Bimake 2x sybr green qpcr master mix utilizes a special performance enhanced taq dna polymerase protected via a hot start activation technique and optimized qpcr buffer system to perform sybr green i based quantitative pcr. The mix prepared at 2x reaction concentration can be directly used for robust and low template quantitative pcr with. Lna enhanced primer sets require specific pcr reaction conditions for optimal performance.

Sybr green master mixes are available with other dyes including rox. Applied biosystems power sybr green pcr master mix delivers highly sensitive nucleic acid quantitation detecting as few as 2 copies of a target gene over a broad range of template concentrations. The jumpstart taq antibody inactivates the dna polymerase at room temperature. This kit contains the high performance mircury sybr green master mix for quantitative real time pcr amplification.

Applied biosystems sybr green master mixes are designed for quantitative real time pcr using a set of two pcr primers that flank the target region. Direct detection of pcr product is monitored by measuring the increase in fluorescence caused by the binding of sybr green dye to double stranded ds dna. Many pcr machines require a passive reference dye. An internal reference dye such as rox corrects well to well optical variations and is used for fluorescent signal normalization.

Sybr green qpcr with the development of thermal cyclers incorporating fluorescent detection pcr has a new innovative application. The newly optimized formulation contains highly purified applied biosystems amplitaq gold dna polymerase ld to offer greater sensitivity. The master mixes contain buffer dntps thermostable hot start dna polymerase and of course sybr green dye everything needed for real time pcr except the sample and pcr primer pair. This ready to use mixture of sybr green i jumpstart taq dna polymerase 99 pure deoxynucleotides and reaction buffer is provided in a 2 concentrate for ease of use.

In routine pcr the critical result is the final quantity of amplicon generated after the process. The detection is based on sybr green which allows quality control of the resulting pcr amplicon by melting curve analysis.